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Zoe pakov svet imdb.cz:Photoreceptor (PR) cells can recover from photic injury through mechanisms that are not well understood. The TRPM1 cation channel is one candidate mechanism. We compared the differential regulation of Na+ and Ca2+ channels on the single PR cell. Our previous studies demonstrated that Na+ channels in PR cells are selectively upregulated following light damage, whereas Ca2+ channels are downregulated. To investigate their roles in regeneration, we now tested the hypothesis that the upregulation of Na+ channels and the downregulation of Ca2+ channels contribute to the recovery from photic injury. Using electrophysiology, we found that two days after light exposure, PRs had higher Na+ channel density (activated by high frequency stimulation) and inactivation (latency to recovery from inactivation), but lower Ca2+ channel density (activated by elevated K+). In contrast, a week after the light exposure, PR cells had reduced Na+ channel density (resting currents) and increased Ca2+ channel density (activated by high K+). Both the Na+ channel density (due to the reduction in Ca2+ channel density) and the inactivation of Na+ channels (due to upregulation of the Na+ channel density) were correlated with PR cell regeneration. Thus, upregulation of Na+ channels together with downregulation of Ca2+ channels support regeneration. We propose that the modulation of Na+ and Ca2+ channels play a role in the cellular processes that are involved in the recovery from light damage in the retina. For example, the PR cells at a certain stage of regeneration can be activated by stimulating multiple Na+ channels (upregulated Na+ channels) and inhibited by stimulating single Ca2+ channels (downregulated Ca2+ channels).Q:
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